Hemorrhagic Fever and Renal Syndrome Using Samples from Patients with Real-Time Reverse Transcriptase PCR Puumala Hantavirus Viremia Diagnosed by
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Puumala hantavirus viremia diagnosed by real-time reverse transcriptase PCR using samples from patients with hemorrhagic fever and renal syndrome.
Puumala virus (PUUV) is the endemic hantavirus in northern Sweden and causes nephropathia epidemica (NE), a milder form of hemorrhagic fever with renal syndrome. There is a need for fast and reliable diagnostics to differentiate the disease from other infections. By aligning virus RNA sequences isolated from 11 different bank voles and one human patient, we designed a real-time reverse transcri...
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1 Puumala hantavirus viremia determined by real-time RT-PCR in patients with 2 hemorrhagic fever with renal syndrome 3 4 Magnus Evander, Irene Eriksson, Lisa Pettersson, Per Juto, Clas Ahlm, 5 Göran Bucht and Annika Allard 6 Department of Virology, Umeå University, Umeå, Sweden 1 ; Department of Infectious 7 Diseases, Umeå University, Umeå, Sweden 2 ; Department of Medical 8 Countermeasures, Di...
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Rodent-borne hantaviruses cause two severe acute diseases: hemorrhagic fever with renal syndrome (HFRS) in Eurasia, and hantavirus pulmonary syndrome (HPS; also called hantavirus cardiopulmonary syndrome [HCPS]) in the Americas. Puumala virus (PUUV) is the most common causative agent of HFRS in Europe. Current routine diagnostic methods are based on serological analyses and can yield inconclusi...
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